| 1. Jimmy Rogers, James A. Lane - Chicago Bound | not rated | 2:40 |
| 2. Muddy Waters - Mannish Boy | not rated | 2:55 |
| 3. Howlin' Wolf, Willie Dixon - Spoonful | not rated | 2:45 |
| 4. Buddy Guy, Willie Dixon - I Cry and Sing The Blues | not rated | 3:16 |
| 5. Jimmy Reed, Jimmy Reed / Mary Reed - Bright Lights, Big City | not rated | 2:40 |
| 6. Howlin' Wolf, Willie Dixon - Evil (is Goin' On) | not rated | 2:55 |
| 7. Little Walter, William Broonzy - Key to the Highway | not rated | 2:46 |
| 8. Koko Taylor, Willie Dixon - Wang Dang Doodle | not rated | 3:01 |
| 9. Howlin' Wolf, Willie Dixon - The Red Rooster | not rated | 2:26 |
| 10. Koko Taylor, Willie Dixon - I Got What It Takes | not rated | 3:08 |
| 11. Otis Rush, Sandy Jones, Jr. - Walking the Back Streets and Crying | not rated | 5:59 |
| 12. Muddy Waters, Willie Dixon - (I'm Your) Hoochie Coochie Man | not rated | 2:51 |
| 13. Lonnie Brooks, Lonnie Brooks - Feel Good Doin' Bad | not rated | 5:36 |
| 14. Bo Diddley - Diddley Daddy | not rated | 2:28 |
| 15. Magic Slim & the Teardrops, Elmore James - Talk to Me, Baby (I Can't Hold Out) (live) | not rated | 6:56 |
| 16. The Paul Butterfield Blues Band, Nick Gravenites - Born in Chicago | not rated | 3:07 |
| 17. The Paul Butterfield Blues Band, Elmore James / adapted by Paul Butterfield - Shake Your Money Maker | not rated | 2:28 |
| 18. Bob Dylan, Bob Dylan - Maggie's Farm | not rated | 3:55 |
| 19. Public Enemy, Carlton Ridenhour / Eric Sadler / Hank Shocklee - Show 'em Watcha Got | not rated | 1:57 |
| 20. Common, Lonnie Lynn / James Yancey - Doo'in'it | not rated | 3:38 |
| 21. The ElectriK Mud Kats, Melivin London / Elias McDaniel / McKinley Morganfield - Mannish Boy | not rated | 5:21 |
| 22. Etta James, Ellington Jordan / Billy Foster - I'd Rather Go Blind | not rated | 2:30 |
| 1:15:18 |
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