| 1. James Carter & The Prisoners, Traditional - Po Lazarus | not rated | 4:30 |
| 2. Harry McClintock, Harry McClintock - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake, Jimmie Davis/Charles Mitchell - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss, Traditional - Down To The River To Pray | not rated | 2:55 |
| 5. Soggy Bottom Boys, Traditional - I Am a Man of Constant Sorrow (radio version) | not rated | 3:10 |
| 6. Chris Thomas King, Nehemiah "Skip" James - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. Norman Blake, Traditional - I Am a Man of Constant Sorrow | not rated | 4:29 |
| 8. The Whites, A.P. Carter/Sharon White - Keep On The Sunny Side | not rated | 3:34 |
| 9. Gillian Welch & Alison Krauss, Albert E. Brumley - I'll Fly Away | not rated | 3:57 |
| 10. Emmylou Harris, Alison Krauss & Gillian Welch, T Bone Burnett/Gillian Welch - Didn't Leave Nobody But the Baby | not rated | 1:57 |
| 11. Sarah, Hannah & Leah Peasall, Maybelle Carter - In the Highways | not rated | 1:36 |
| 12. The Cox Family, Pete Roberts - I Am Weary (Let Me Rest) | not rated | 3:14 |
| 13. John Hartford, Traditional - I Am a Man of Constant Sorrow | not rated | 2:34 |
| 14. Ralph Stanley, Traditional - O Death - () | not rated | 3:19 |
| 15. Soggy Bottom Boys & Tim Blake Nelson, Jimmie Rodgers - In the Jail House Now | not rated | 3:36 |
| 16. Soggy Bottom Boys, Traditional - I Am a Man of Constant Sorrow (band version) | not rated | 4:16 |
| 17. John Hartford, Hoyt Ming/John Hartford - Indian War Whoop | not rated | 1:30 |
| 18. Fairfield Four, Traditional/Isaac Freeman - Lonesome Valley | not rated | 4:07 |
| 19. The Stanley Brothers, Traditional - Angel Band | not rated | 2:17 |
| 1:00:36 |
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